Conformational change Contents Laboratory analysis See also External links References Navigation menuFrauenfelder, H. New looks at protein motions Nature 338, 623 - 624 (20 April 1989)Sensing with electro-switchable biosurfacesBiodesy10.1021/jp803703m18928314expanding ite

Microbiology techniquesProtein stubs


biochemistrymacromoleculepHvoltagelightchromophoresionphosphorylationligandcrystallographyNMRelectron paramagnetic resonancespin labelcircular dichroism (CD)hydrogen exchangeFRETDual polarisation interferometryelectro-switchable biosurfaces




Main article: Protein dynamics

In biochemistry, a conformational change is a change in the shape of a macromolecule, often induced by environmental factors.


A macromolecule is usually flexible and dynamic. It can change its shape in response to changes in its environment or other factors; each possible shape is called a conformation, and a transition between them is called a conformational change. Factors that may induce such changes include temperature, pH, voltage, light in chromophores, ion concentration, phosphorylation, or the binding of a ligand.




Contents





  • 1 Laboratory analysis


  • 2 See also


  • 3 External links


  • 4 References




Laboratory analysis


Many biophysical techniques such as crystallography, NMR, electron paramagnetic resonance (EPR) using spin label techniques, circular dichroism (CD), hydrogen exchange, and FRET can be used to study macromolecular conformational change. Dual polarisation interferometry is a benchtop technique capable of measuring conformational changes in biomolecules in real time at very high resolution.


A specific nonlinear optical technique called second-harmonic generation (SHG) has been recently applied to the study of conformational change in proteins.[1] In this method, a second-harmonic-active probe is placed at a site that undergoes motion in the protein by mutagenesis or non-site-specific attachment, and the protein is adsorbed or specifically immobilized to a surface. A change in protein conformation produces a change in the net orientation of the dye relative to the surface plane and therefore the intensity of the second harmonic beam. In a protein sample with a well-defined orientation, the tilt angle of the probe can be quantitatively determined, in real space and real time. Second-harmonic-active unnatural amino acids can also be used as probes.


Another method applies electro-switchable biosurfaces where proteins are placed on top of short DNA molecules which are then dragged through a buffer solution by application of alternating electrical potentials. By measuring their speed which ultimately depends on their hydrodynamic friction, conformational changes can be visualized.



See also


  • Database of protein conformational diversity

  • Protein dynamics

  • The Database of Macromolecular Motions (molmovdb)


External links



  • Frauenfelder, H. New looks at protein motions Nature 338, 623 - 624 (20 April 1989).

  • Sensing with electro-switchable biosurfaces

  • Biodesy


References




  1. ^ Salafsky, Joshua S.; Cohen, Bruce (2008). "A Second-Harmonic-Active Unnatural Amino Acid as a Structural Probe of Biomolecules on Surfaces". Journal of Physical Chemistry. 112 (47): 15103–15107. doi:10.1021/jp803703m. PMID 18928314..mw-parser-output cite.citationfont-style:inherit.mw-parser-output .citation qquotes:"""""""'""'".mw-parser-output .citation .cs1-lock-free abackground:url("//upload.wikimedia.org/wikipedia/commons/thumb/6/65/Lock-green.svg/9px-Lock-green.svg.png")no-repeat;background-position:right .1em center.mw-parser-output .citation .cs1-lock-limited a,.mw-parser-output .citation .cs1-lock-registration abackground:url("//upload.wikimedia.org/wikipedia/commons/thumb/d/d6/Lock-gray-alt-2.svg/9px-Lock-gray-alt-2.svg.png")no-repeat;background-position:right .1em center.mw-parser-output .citation .cs1-lock-subscription abackground:url("//upload.wikimedia.org/wikipedia/commons/thumb/a/aa/Lock-red-alt-2.svg/9px-Lock-red-alt-2.svg.png")no-repeat;background-position:right .1em center.mw-parser-output .cs1-subscription,.mw-parser-output .cs1-registrationcolor:#555.mw-parser-output .cs1-subscription span,.mw-parser-output .cs1-registration spanborder-bottom:1px dotted;cursor:help.mw-parser-output .cs1-ws-icon abackground:url("//upload.wikimedia.org/wikipedia/commons/thumb/4/4c/Wikisource-logo.svg/12px-Wikisource-logo.svg.png")no-repeat;background-position:right .1em center.mw-parser-output code.cs1-codecolor:inherit;background:inherit;border:inherit;padding:inherit.mw-parser-output .cs1-hidden-errordisplay:none;font-size:100%.mw-parser-output .cs1-visible-errorfont-size:100%.mw-parser-output .cs1-maintdisplay:none;color:#33aa33;margin-left:0.3em.mw-parser-output .cs1-subscription,.mw-parser-output .cs1-registration,.mw-parser-output .cs1-formatfont-size:95%.mw-parser-output .cs1-kern-left,.mw-parser-output .cs1-kern-wl-leftpadding-left:0.2em.mw-parser-output .cs1-kern-right,.mw-parser-output .cs1-kern-wl-rightpadding-right:0.2em








Microbiology techniques, Protein stubsUncategorized
-

Popular posts from this blog

Frič See also Navigation menuinternal link

Image
SurnamesCzech-language surnames ‹ The template Infobox surname is being considered for merging. › Frič Origin Language(s) Czechized German (eastern Middle German dialects and Alemannic German) - Thuringian, Upper Saxon, Low Lusatian, Silesian Meaning derived from German: Friedrich Other names Variant(s) Fritsch, Fritzsch, Frycz, Fricz, Fryczyński, Frietsch, Fritsche, Fritzsche; Fritschi (Alemannic), Fritz, Fritze, Fritzel, Fritzl, Fritzke, Fritzen Frič is a Czechized German surname. Notable people with the surname include: Alberto Vojtěch Frič, Czech botanist Antonín Jan Frič (Fritsch) , Czech paleontologist Jaroslav Erik Frič, Czech poet Martin Frič, Czech film director, screenwriter and actor See also Fričovce (Hungarian: Frics ) .mw-parser-output table.dmboxclear:both;margin:0.9em 1em;border-top:1px solid #ccc;border-bottom:1px solid #ccc;background-color:transparent Surname list This page lists people with...
Read more

Identify plant with long narrow paired leaves and reddish stems Planned maintenance scheduled April 17/18, 2019 at 00:00UTC (8:00pm US/Eastern) Announcing the arrival of Valued Associate #679: Cesar Manara Unicorn Meta Zoo #1: Why another podcast?What is this plant with long sharp leaves? Is it a weed?What is this 3ft high, stalky plant, with mid sized narrow leaves?What is this young shrub with opposite ovate, crenate leaves and reddish stems?What is this plant with large broad serrated leaves?Identify this upright branching weed with long leaves and reddish stemsPlease help me identify this bulbous plant with long, broad leaves and white flowersWhat is this small annual with narrow gray/green leaves and rust colored daisy-type flowers?What is this chilli plant?Does anyone know what type of chilli plant this is?Help identify this plant

Image
Apollo command module space walk? prime numbers and expressing non-prime numbers Can a non-EU citizen traveling with me come with me through the EU passport line? Extract all GPU name, model and GPU ram How to deal with a team lead who never gives me credit? Why was the term "discrete" used in discrete logarithm? Why do we bend a book to keep it straight? When do you get frequent flier miles - when you buy, or when you fly? How come Sam didn't become Lord of Horn Hill? At the end of Thor: Ragnarok why don't the Asgardians turn and head for the Bifrost as per their original plan? Is it true that "carbohydrates are of no use for the basal metabolic need"? Using et al. for a last / senior author rather than for a first author How to tell that you are a giant? How to react to hostile behavior from a senior developer? String `!23` is replaced with `docker` in command line How to find out what spells would be useless to a blind NPC sp...
Read more

fontconfig warning: “/etc/fonts/fonts.conf”, line 100: unknown “element blank” The 2019 Stack Overflow Developer Survey Results Are In“tar: unrecognized option --warning” during 'apt-get install'How to fix Fontconfig errorHow do I figure out which font file is chosen for a system generic font alias?Why are some apt-get-installed fonts being ignored by fc-list, xfontsel, etc?Reload settings in /etc/fonts/conf.dTaking 30 seconds longer to boot after upgrade from jessie to stretchHow to match multiple font names with a single <match> element?Adding a custom font to fontconfigRemoving fonts from fontconfig <match> resultsBroken fonts after upgrading Firefox ESR to latest Firefox

Image
How to type this arrow in math mode? Multiply Two Integer Polynomials What do hard-Brexiteers want with respect to the Irish border? Why isn't the circumferential light around the M87 black hole's event horizon symmetric? For what reasons would an animal species NOT cross a *horizontal* land bridge? Can one be advised by a professor who is very far away? Identify boardgame from Big movie slides for 30min~1hr skype tenure track application interview Why do we hear so much about the Trump administration deciding to impose and then remove tariffs? If I score a critical hit on an 18 or higher, what are my chances of getting a critical hit if I roll 3d20? Can we generate random numbers using irrational numbers like π and e? Worn-tile Scrabble Output the Arecibo Message What is the meaning of Triage in Cybersec world? How are circuits which use complex ICs normally simulated? Shouldn't "much" here be used instead of "more"? Dele...
Read more